国际生殖健康/计划生育杂志

国际生殖健康/计划生育 ›› 2013, Vol. 32 ›› Issue (6): 478-480.

• 综述 • 上一篇    下一篇

雄性生殖细胞冷冻保存研究进展

王 丽综述 姜 宏   

  1. 230031 合肥，安徽医科大学解放军临床学院生殖医学中心
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2013-11-15 发布日期:2013-11-15
  • 通讯作者: 姜 宏

Progress on the Cryopreservation of Male Germ Cell

WANG Li，JIANG Hong   

  1. Reproductive Medical Center，Clinical College of People′s Liberation Army，Anhui Medical University，Hefei 230031，China
  • Received:1900-01-01 Revised:1900-01-01 Published:2013-11-15 Online:2013-11-15
  • Contact: JIANG Hong

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摘要： 随着单精子及精子细胞卵胞浆内显微受精技术的发展，雄性生殖细胞冷冻保存技术已成为辅助生殖技术的重要内容，为男性因素不育患者的临床助孕提供了物质保障基础。目前，雄性生殖细胞冷冻方法包括程序化冷冻和玻璃化冷冻；冷冻保护剂可有效降低细胞的冷冻损伤，根据其特性分为渗透性保护剂和非渗透性保护剂两大类；冷冻载体中冷冻环、冷冻叶片等应用较广泛。雄性生殖细胞冷冻的效果与冷冻方法、冷冻保护剂和冷冻载体等密切相关。

关键词: 低温保存, 精液保存, 精子, 精细胞, 精子注射, 细胞质内, 玻璃化冷冻, 冷冻载体, 冷冻保护剂

Abstract: Following the development of intracytoplasmic sperm and haploid spermatid injection techniques，the cryopreservation technology of male germ cell, as important part of assisted reproductive technology (ART), has played a key role in the ART treatment for the couples with male infertility. Freezing methods of male germ cells include the programmed cryopreservation and vitrification freezing. Cryoprotectant，usually classified as permeable and impermeable cryoprotective agents，could minimize the cell injuries during the freeze-thaw. Various frozen carriers such as cryoloop and cryoleaf are widely used in cell cryopreservation. In this paper，the freezing method，carrier，cryoprotectants and frozen effect of male germ cell were reviewed.

Key words: Cryopreservation, Semen preservation, Spermatozoa, Spermatids, Sperm injections, intracytoplasmic, Vitrification, Frozen carrier, Cryoprotectant