国际生殖健康/计划生育 ›› 2015, Vol. 34 ›› Issue (2): 141-144.

• 综述 • 上一篇    下一篇

卵巢组织冻存方案的优化研究进展

曾玉翠,吴瑞芳   

  1. 518036 广东省深圳市,北京大学深圳医院妇产科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2015-03-15 发布日期:2015-03-15

Progress on Optimal Protocols of Ovarian Tissue Cryopreservation

ZENG Yu-cui,WU Rui-fang   

  1. Department of Obstetrics and Gynecology,Beijing University Shenzhen Hospital,Shenzhen 518036,Guangdong Province,China
  • Received:1900-01-01 Revised:1900-01-01 Published:2015-03-15 Online:2015-03-15

摘要: 随着肿瘤治疗技术的提高,保护女性生育力逐渐引起了人们的关注。胚胎冷冻技术在各大生殖中心的运用使女性患者一定程度获益,但胚胎冷冻应用的局限性使卵巢组织冷冻成为研究的热点,而冷冻方法的研究成为第一难题。目前针对卵巢组织块的冷冻方法主要包括慢速程序化冷冻和玻璃化冷冻。相比卵巢组织块的冷冻方法,定向冷冻的应用在整个卵巢冷冻中更有优势。定向冷冻能使整个卵巢的冷却速度保持一致,从而降低整个卵巢在冷冻过程中的损伤。不同的冷冻方法对卵巢组织冷冻和(或)解冻移植后功能恢复的影响程度不同,因此寻找合适的冷冻方法对保证最佳冻存效率有着重要的意义。综述近期优化的卵巢组织冻存方案,为临床应用提供参考。

关键词: 生育力, 卵巢, 组织保存, 低温保存

Abstract: As the advances in treatment of neoplasia, the protection of female fertility is attaching more and more attention. The application of embryo cryopreservation in IVF center brings definite benefits to some patients. However, there are limitations in embryo cryopreservation. The ovarian tissue cryopreservation is a new research area, in which the cryopreservation technology is the first problem. Two ways of ovarian tissue cryopreservation are slow freezing and vitrification. Compared with the freezing ways using in the piece of ovarian tissue,the directional freezing is a preponderant method for the whole ovary. The directional freezing can keep the whole ovary at the same cooling rate, which is thought to reduce the injury during the whole cooling process. Different freezing protocols have different effects on functional recovery of the frozen-thawed ovarian tissue. It is important to explore the suitable and standard protocol to optimize the efficiency of cryopreservation. Those optimal protocols of ovarian tissue cryopreservation were discussed in this review, so as to provide some references for clinical practice.

Key words: Fertility, Ovary, Tissue preservation, Cryopreservation