Abstract: The male fertility is affected by many factors and sperm DNA damage is one of the hot
spots in ART. Spermatozoa mitochondria and nucleus DNA damage have negative effects on normal fertilized
process，pronucleus shaping，implantation and offspring health. At present，sperm DNA damage can be
detected by more and more methods, although none of these provides an ideal method to assess sperm DNA
integrity, and each has its clinical application，which are more stable and more sensitive than the conventional
analysis of semen. It is necessary to assess sperm DNA damage before ICSI. Assessment of sperm DNA damage
is considered to be a new evaluation of semen quality and fertility prediction indicators. This article reviews the
studies on detection of sperm DNA damage and association with reproductive outcomes.

Key words: INFERTILITY, MALE, Spermatozoa, DNA, mitochondrial, Semen, Reproductive techniques, assisted, Pregnancy outcome