Abstract: Objective： To develop a rat model of polycystic ovarian syndrome（PCOS） with type 2 diabetes（2-DM） induced by sodium prasterone sulfate and streptozotocin（STZ）. Methods： Female rats aged 23-day were injected daily S. C. with sodium prasterone sulfate 90 μg/（g·d） for 20 days, then intraperitoneally injected streptozotocin once in a dose of 45 mg/kg. All rats were feed with high fat and sugar feed. The ovarian weight and pathological morphology was observed. The sera levels of testosterone（T），estradiol（E2）， luteinizing hormone（LH）， follicle-stimulating hormone（FSH）， blood glucose（BG） and insulin（INS） were measured. Results： The ovaries of both PCOS group and the PCOS with 2-DM group showed multiple follicular cysts. Ovarian weight, sera T, E2 and INS of two groups were significantly higher than those of control group（P＜0.01）. BG concentration of the PCOS with 2-DM group was significantly higher than those of PCOS group and control group（P＜0.01）， while HOMA index of the PCOS with 2-DM group was higher that that of control group（P＜0.05）. Conclusions： The rat model of PCOS with 2-DM showed the morphologic and hormonal changes which were similar to patients with PCOS with 2-DM. This animal model induced by the sodium prasterone sulfate and streptozotocin is a good model of PCOS with 2-DM for studying the long?鄄term complications of PCOS.

Key words: Polycystic ovary syndrome, Diabetes mellitus, type 2, Dehydroepiandrosterone sulfate, Diabetes mellitus, experimental, Disease models, animal