Abstract: It is important to assay anti-Müllerian hormone (AMH) in the field of assisted reproduction. AMH level can be used to assess the ovarian reserve function, and to predict ovarian response, which provides clinicians the basis to develop an individual treatment so as to reduce the ovarian hyperstimulation syndrome (OHSS) rate and to raise the oocyte-obtained rate. With 20 years′ development, AMH test has been developed from the manual ELISA, the automated ELISA, to the electrochemical luminescence method, and the test reagents have also undergone several generations of development. There are differences in the efficiency of AMH test in different methods and reagents, or the same method and different reagents. If the same method and reagent were used, the main factor of the AMH test differences should be the sample itself. In addition, some non-experimental factors could affect the objectivity of AMH test, such as ovarian function, menstrual cycle, pituitary tone, ovulation and so on. This article summarized those experimental factors and non-experimental factors related to AMH test.

Key words:  Anti-Mullerian hormone, Enzyme-linked immunosorbent assay, Diagnostic techniques, endocrine, Fertility, Reproductive techniques, assisted