卵巢皮质玻璃化冷冻保存及移植的损伤因素

doi:10.12280/gjszjk.20220115

摘要/Abstract

摘要：

卵巢组织冻存和移植在技术上可行,有效性也随着技术发展而提高,可用于女性生育力保存,尤其是青春期前放化疗的患癌女性的生育能力保存。玻璃化冷冻被认为是目前最有潜力的卵巢组织冻存方法之一,但其安全性和有效性尚需进一步验证。冷冻损伤及移植缺血再灌注损伤是卵巢冻存和移植中需要克服的难题,如何尽可能有效地保存卵泡是难点及重点。从冷冻保护剂、卵巢组织大小、冷冻载体、抗氧化剂、促进新血管形成和激素6个方面对目前已有的玻璃化冷冻及移植条件、效果等进行了阐述,有益于女性生育力保存的项目推进。

关键词: 卵巢, 玻璃, 低温保存, 保存,生物学, 组织移植, 再灌注损伤

Abstract:

The cryopreservation and transplantation of ovarian tissue is technically feasible, and the effectiveness is improved with the development of technology. It can be used to preserve female fertility, especially to preserve the fertility of prepubertal women with cancer who will have radiotherapy and chemotherapy. Vitrification is considered to be one of the most promising methods for cryopreservation of ovarian tissue, but its safety and efficacy need to be further verified. Cryogenic injury and transplantation ischemia-reperfusion injury are two problems of further development of cryopreservation and transplantation, in which the difficulty and focus are how to preserve follicles effectively. The existing freezing and transplantation conditions and effects are evaluated from six aspects of cryoprotectant, ovarian tissue size, cryocarrier, antioxidants, promoting angiogensis and hormones, in order to advance the project of female fertility preservation.

Key words: Ovary, Glass, Cryopreservation, Preservation, biological, Tissue transplantation, Reperfusion injury

黄少凤, 牛向丽, 林忠, 朱雪红, 宾力, 刘姝岑. 卵巢皮质玻璃化冷冻保存及移植的损伤因素[J]. 国际生殖健康/计划生育, 2022, 41(4): 308-312.

HUANG Shao-feng, NIU Xiang-li, LIN Zhong, ZHU Xue-hong, BIN Li, LIU Shu-cen. Injury Factors of Vitrification Cryopreservation and Transplantation of Ovarian Cortex[J]. Journal of International Reproductive Health/Family Planning, 2022, 41(4): 308-312.

参考文献 34

[1]	Rozen G, Avagliano S, Agresta F, et al. Ovarian tissue grafting: Lessons learnt from our experience with 55 grafts[J]. Reprod Med Biol, 2021, 20(3):277-288. doi: 10.1002/rmb2.12380. doi: 10.1002/rmb2.12380 URL
[2]	Cacciottola L, Donnez J, Dolmans MM. Ovarian tissue and oocyte cryopreservation prior to iatrogenic premature ovarian insufficiency[J]. Best Pract Res Clin Obstet Gynaecol, 2022, 81:119-133. doi: 10.1016/j.bpobgyn.2021.09.010. doi: 10.1016/j.bpobgyn.2021.09.010 URL
[3]	Liebenthron J, Montag M, Reinsberg J, et al. Overnight ovarian tssue transportation for centralized cryobanking:a feasible option[J]. Reprod Biomed Online, 2019, 38(5):740-749. doi: 10.1016/j.rbmo.2019.01.006. doi: S1472-6483(19)30054-9 pmid: 30733076
[4]	Youm HS, Choi JR, Oh D, et al. Vitrfication and slow freezing for cryopreservation of germinal vesicle-stage human oocytes: a bayesian Meta-analysis[J]. Cryo Letters, 2017, 38(6):455-462.
[5]	Pimentel MM, Dos Santos FA, et al. Rescue of caprine fetal ovaries,vitrification and follicular development after xenotransplantation in two immunodeficient mice models[J]. Anim Reprod, 2020, 17(2):e20190115. doi: 10.1590/1984-3143-AR2019-0115. doi: 10.1590/1984-3143-AR2019-0115 URL
[6]	高妍, 吕岩, 官员, 等. ε-聚赖氨酸对小鼠胚胎玻璃化冷冻的影响[J]. 中国兽医学报, 2020, 40(6):167-170. doi: 10.16303/j.cnki.1005-4545.2020.06.28. doi: 10.16303/j.cnki.1005-4545.2020.06.28
[7]	Leonel ECR, Corral A, Risco R, et al. Stepped vitrification technique for human ovarian tissue cryopreservation[J]. Sci Rep, 2019, 9(1):20008. doi: 10.1038/s41598-019-56585-7. doi: 10.1038/s41598-019-56585-7 URL
[8]	Obata R, Nakumura Y, Okuyama N, et al. Comparison of Residual Dimethyl Sulfoxide (DMSO) and Ethylene Glycol (EG) Concentration in Bovine Ovarian Tissue During Warming Steps Between Slow Freezing and Vitrification[J]. Cryo Letters, 2018, 39(4):251-254.
[9]	Wiweko B, Soebijanto S, Boediono A, et al. Survival of isolated human preantral follicles after vitrification: Analyses of morphology and Fas ligand and caspase-3 mRNA expression[J]. Clin Exp Reprod Med, 2019, 46(4):152-165. doi: 10.5653/cerm.2019.00143. doi: 10.5653/cerm.2019.00143 pmid: 31813207
[10]	Gandolfi F, Paffoni A, Papasso BE, et al. Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models[J]. Fertil Steril, 2006, 85(1):1150-1156. doi: 10.1016/j.fertnstert.2005.08.062. doi: 10.1016/j.fertnstert.2005.08.062 URL
[11]	Fabbri R, Vicenti R, Macciocca M, et al. Morphological,ultrastructural and functional imaging of frozen/thawed and vitrified/warmed human ovarian tissue retrieved from oncological patients[J]. Hum Reprod, 2016, 31(8):1838-1849. doi: 10.1093/humrep/dew134. doi: 10.1093/humrep/dew134 URL
[12]	Corral A, Clavero M, Gallardo M, et al. Ovarian tissue cryopreservation by stepped vitrification and monitored by X-ray computed tomography[J]. Cryobiol, 2018, 81(1): 17-26. doi: 10.1016/j.cryobiol.2018.03.001. doi: 10.1016/j.cryobiol.2018.03.001 URL
[13]	Zhao Q, Zhang Y, Su K, et al. Vitrification freezing of large ovarian tissue in the human body[J]. J Ovarian Res, 2019, 12(1):77. doi: 10.1186/s13048-019-0553-x. doi: 10.1186/s13048-019-0553-x URL
[14]	Gavish Z, Peer G, Roness H, et al. Follicle activation and ′burn-out′ contribute to post-transplantation follicle loss in ovarian tissue grafts: the effect of graft thickness[J]. Hum Reprod, 2014, 29(5):989-996. doi: 10.1093/humrep/deu015. doi: 10.1093/humrep/deu015 URL
[15]	Haino T, Tarumi W, Kawamura K, et al. Determination of Follicular Localization in Human Ovarian Cortex for Vitrification[J]. J Adolesc Young Adult Oncol, 2018, 7(1):46-53. doi: 10.1089/jayao.2017.0028. doi: 10.1089/jayao.2017.0028 URL
[16]	杨丽, 张曜曜, 李灵玲, 等. 银制封闭式冷冻载体改善人卵巢组织异种移植效果的研究[J]. 实用妇产科杂志, 2018, 34(9):686-689.
[17]	Terren C, Fransolet M, Ancion M, et al. Slow Freezing Versus Vitrification of Mouse Ovaries: from Ex Vivo Analyses to Successful Pregnancies after Auto-Transplantation[J]. Sci Rep, 2019, 9(1):19668. doi: 10.1038/s41598-019-56182-8. doi: 10.1038/s41598-019-56182-8 URL
[18]	肖准, 汪燕, 李灵玲, 等. 新型玻璃化冷冻法在人卵巢组织深低温保存的应用[J]. 实用妇产科杂志, 2013, 29(12):928-931.
[19]	Donnez J, Dolmans MM, Demylle D, et al. Livebirth after orthotopic transplantation of cryopreserved ovarian tissue[J]. Lancet, 2004, 364(9443):1405-1410. doi: 10.1016/S0140-6736(04)17222-X. doi: 10.1016/S0140-6736(04)17222-X pmid: 15488215
[20]	孙宁霞, 李紫袁, 庞文娟, 等. 中国首例卵巢组织冷冻移植活产2年随访[J]. 中华生殖与避孕杂志, 2021, 41(11):1026-1030. doi: 10.3760/cma.j.cn101441-20210719-00312. doi: 10.3760/cma.j.cn101441-20210719-00312
[21]	杨璐凯, 蒋利刚, 崔妍婷, 等. 槲皮素在羊卵巢组织玻璃化冻存中的卵泡保护及抗氧化作用[J]. 山东大学学报(医学版), 2020, 58(9):1-7. doi: 10.6040/j.issn.1671-7554.0.2020.0655. doi: 10.6040/j.issn.1671-7554.0.2020.0655
[22]	Wang D, Geng M, Gan D, et al. Effect of resveratrol on mouse ovarian vitrification and transplantation[J]. Reprod Biol Endocrinol, 2021, 19(1):54. doi: 10.1186/s12958-021-00735-y. doi: 10.1186/s12958-021-00735-y URL
[23]	Palhares PC, Assis IL, Machado GJ, et al. Sperm characteristics,peroxidation lipid and antioxidant enzyme activity changes in milt of Brycon orbignyanus cryopreserved with melatonin in different freezing curves[J]. Theriogenology, 2021, 176:18-25. doi: 10.1016/j.theriogenology.2021.09.013. doi: 10.1016/j.theriogenology.2021.09.013 URL
[24]	Kong HS, Hong YH, Lee J, et al. Antifreeze Protein Supplementation During the Warming of Vitrified Bovine Ovarian Tissue Can Improve the Ovarian Tissue Quality After Xenotransplantation[J]. Front Endocrinol(Lausanne), 2021, 12:672619. doi: 10.3389/fendo.2021.672619. doi: 10.3389/fendo.2021.672619
[25]	Kim MK, Kong HS, Youm HW, et al. Effects of supplementation with antifreeze proteins on the follicular integrity of vitrified-warmed mouse ovaries: Comparison of two types of antifreeze proteins alone and in combination[J]. Clin Exp Reprod Med, 2017, 44(1):8-14. doi: 10.5653/cerm.2017.44.1.8. doi: 10.5653/cerm.2017.44.1.8 URL
[26]	Fransolet M, Noël L, Henry L, et al. Evaluation of Z-VAD-FMK as an anti-apoptotic drug to prevent granulosa cell apoptosis and follicular death after human ovarian tissue transplantation[J]. Assist Reprod Genet, 2019, 36(2):349-359. doi: 10.1007/s10815-018-1353-8. doi: 10.1007/s10815-018-1353-8 URL
[27]	Kong HS, Lee J, Youm HW, et al. Effect of treatment with angiopoietin-2 and vascular endothelial growth factor on the quality of xenografted bovine ovarian tissue in mice[J]. PLoS One, 2017, 12(9):e0184546. doi: 10.1371/journal.pone.0184546. doi: 10.1371/journal.pone.0184546 URL
[28]	Abir R, Fisch B, Fisher N, et al. Attempts to improve human ovarian transplantation outcomes of needle-immersed vitrification and slow-freezing by host and graft treatments[J]. J Assist Reprod Genet, 2017, 34(5):633-644. doi: 10.1007/s10815-017-0884-8. doi: 10.1007/s10815-017-0884-8 URL
[29]	Lee S, Cho HW, Kim B, et al. The Effectiveness of Anti-Apoptotic Agents to Preserve Primordial Follicles and Prevent Tissue Damage during Ovarian Tissue Cryopreservation and Xenotransplantation[J]. Int J Mol Sci, 2021, 22(5):2534. doi: 10.3390/ijms22052534. doi: 10.3390/ijms22052534 URL
[30]	Gartrell BA, Tsao CK, Galsky MD. The follicle-stimulating hormone receptor:a novel target in genitourinary malignancies[J]. Urol Oncol, 2013, 31(8):1403-1407. doi: 10.1016/j.urolonc.2012.03.005. doi: 10.1016/j.urolonc.2012.03.005 URL
[31]	陈杰, 王燕蓉, 常青, 等. 促卵泡刺激素对小鼠卵巢玻璃化冻存的抗凋亡作用[J]. 中国应用生理学杂志, 2018, 34(6):540-542. doi: 10.12047/j.cjap.5689.2018.120. doi: 10.12047/j.cjap.5689.2018.120
[32]	Kang BJ, Wang Y, Zhang L, et al. bFGF and VEGF improve the quality of vitrified-thawed human ovarian tissues after xenotransplantation to SCID mice[J]. J Assist Reprod Genet, 2016, 33(2):281-289. doi: 10.1007/s10815-015-0628-6. doi: 10.1007/s10815-015-0628-6 URL
[33]	陈杰, 王燕蓉, 常青, 等. 玻璃化冻融不同时段rFSH联合rLH干预对小鼠卵巢VEGF及Cx37表达的影响[J]. 宁夏医科大学学报, 2019, 41(2):114-120. doi: 10.16050/j.cnki.issn1674-6309.2019.02.002. doi: 10.16050/j.cnki.issn1674-6309.2019.02.002
[34]	Abbara A, Clarke SA, Dhillo WS. Novel Concepts for Inducing Final Oocyte Maturation in In Vitro Fertilization Treatment[J]. Endocr Rev, 2018, 39(5):593-628. doi: 10.1210/er.2017-00236. doi: 10.1210/er.2017-00236 pmid: 29982525