Abstract: Objective：To investigate the correlation between the insulin gene variable number tandem
repeats（INS鄄VNTR）and polycystic ovary syndrome（PCOS）and metabolic features related to insulin resistance
（IR）. Methods：One hundred and thirty patients with PCOS（PCOS group）and 130 normal women（control
group）were included. Genotyping of INS-VNTR was performed using polymerase chain reaction-restriction
fragment length polymorphism（PCR-RFLP）. Results：The distribution of genotype of INS-VNTR was similar in
PCOS group，and compared with the I/Icarriers, an odd ratio （OR）for III/IIIcarriers was 6.25 [95%
confidence interval（CI）=0.749-52.79]. The distribution of genotype and the 芋allele frequency of INS-VNTR
in PCOS insulin resistance（PCOS-IR）group were significantly higher than that in PCOS non-insulin resistance
group （PCOS-NIR）. Moreover, PCOS women with III/IIIhad statistically significantly higher fasting insulin
lever and HOMA-IR than that of PCOS women with I/I. Conclusions：INS-VNTR may not be a susceptibility
gene，but INS VNTR polymorphism may play an important role in the occurrence of insulin resistance in
patients with PCOS.

Key words: Polycystic ovary syndrome, Insulin, Polymorphism, genetic, Insulin resistance, Polymerase chain reaction, Polymorphism, restriction fragment length, Tandem repeat sequences