Abstract: Objective： To evaluate the diagnostic application of the high-risk human papilloma virus DNA （HPV-DNA） detection combined with liquid-based cytology technique （LCT） in cervical precancerous lesion. Methods：365 women with cervical precancerous lesion from Department of obstetrics and gynecology，Jiangsu Cancer Hospital，January 2008 to March 2010，were selected. The Screening of cervical cancer was performed by LCT and HPV-DNA detection. All women underwent colposcopic cervical biopsies and pathological examination. Pathological examination was regard as the gold standard to diagnosis cervical precancerous lesions. Results：The HPV positive results were observed in 106 cases and the LCT positive results （≥ASCUS） in 178 cases，the positive results of pathological examination were 84 and 121 cases，respectively. In 143 women with negative results of LCT and HPV detection，only two cases had the posotive results of pathological examination. The diagnostic sensitivity and specificity of LCT for cervical precancerous lesions were 80.1% and 84.9%. The diagnostic sensitivity and specificity of HPV were 55.6% and 74.1%. The sensitivity and specificity increased to 98.7% and 98.6% when combined two methods. Conclusions：Liquid-based cytology technique combined with high-risk HPV-DNA detection is better than any single technique for screening precancerous lesion of cervical carcinoma.

Key words: Precancerous conditions, Uterine cervical neoplasms, DNA probes, HPV, Cytodiagnosis