Abstract: Mammalian target of rapamycin（mTOR）, one member of the family of phosphatidylinositol kinase, is a kind of highly conservative Ser/Thr protein kinase. It regulates many physiological functions, such as protein synthesis，metabolism and oxidative stress，which plays an important role in cell proliferation，growth and differentiation. It was confirmed that mTOR signaling pathway can regulate the proliferation and differentiation of Sertoli cell. The mTOR signaling pathway regulates the formation of blood-testis barrier，but also mTORC1 and mTORC2 respectively regulate the lock and open of blood-testis barrier. The mTOR pathway regulates proliferation and self-renewal of spermatogenia stem cell，and the meiosis of spermatocyte in the process of spermatogenesis. The review discussed the effects of mTOR signaling pathway in regulating the proliferation of sertoli cell，the formation of blood-testis barrier and spermatogenesis.

Key words: Mammalian target of rapamycin, Signal transduction, Spermatogenesis, Sertoli cells, Blood-testis barrier